FIG. 7.

Skin fibroblast (FB) does not alter DC activation. DC were cocultured for 24 hr with either MSC or primary skin FB at 1:10 ratios (MSC:DC or FB:DC). DC were then collected, and the relative surface expression of (A) MHCII, (B) CD86, or (C) CD40 was determined by flow cytometry. Results shown are representative of three separate experiments. Cytometry events were gated on cells expressing high levels of CD11c, and values are given as geometric MFI. P values calculated for statistical variance were determined using a paired two-tailed Mann–Whitney test. *P < 0.05, **P < 0.01, and ***P < 0.005.