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DAPI fluorescence reflects the nuclear density at time points indicated above the image (A, E, I, M, D, Q). GFP fluorescence is also relatively similar (B, F, J, N, R) while immunoreactivity to Nestin, neural stem cell marker (red) is diminished over time (C, G, K), similarly anti-Ki67 immunoreactivity diminished with time (O, S). The bottom panel shows the combined fluorescence of GFP and Nestin (D, H, L) or GFP and Ki67 (P, T). (Scale bar 10 μm.)
