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The first, second, and third columns show GFP (A, D, G), antibody (B, E ,H) and combined fluorescence (C, F, I) signal respectively. At 16 weeks post-transplantation, transplant cells do not express astrocytic marker, GFAP as evident by absence of overlap between GFP and GFAP (red) fluorescence (C). GFP positive transplant cells do not express oligodendrocytic precursor marker, Olig2 (F) or mature oligodendrocyte marker myelin basic protein (MBP) (I). (Scale bar 10 μm.)
