FIG. 6.

Distribution of green fluorescent protein (GFP) cells and processes. Examples of transplant-derived cells and processes in the host parenchyma. Pyramidal-shaped cell in the cortex (A), hippocampus cornus ammonis 1 (CA1) (B), and thalamus (C) revealed by GFP and 2-(4-amidinophenyl)-1H-indole-6-carboxamidine (DAPI) (blue) fluorescence. Cells in the thalamus appear to extend processes along the white matter tracts of the internal capsule (IC) (dotted outline in D). A sagittal section shows the brainstem and cerebellum in E. Higher magnification of the boxes F and G show the presence of GFP fibers in the corresponding higher resolution image on the right. The tip of a GFP process with a growth cone morphology is shown in H. μm bar is 10 μm for A–C, 1000 μm for D, and 10 μm for F–H. Confocal image of penetrating ballistic-like brain injury (PBBI) brain section with GFP+ human neural stem cell grafts (NSCs) at week 8 post-transplantation shows the distribution of transplant and GFP processes (image at center). White boxes in the image are shown at a higher magnification in a counterclockwise arrangement. The GFP+ transplant-derived processes bilaterally wrap the thalamus. GFP processes from the transplant (I) cross the posterior commissure (J) contralateral thalamic surface (contralateral thalamus, K) with the lateral ventricle (LV), culminating ventrally by the internal capsule (L). GFP cells and processes can be seen in the periaqueductal gray (M) and ipsilateral internal capsule (N). μm bar is 30 μm in K–N.