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. Author manuscript; available in PMC: 2019 Dec 16.
Published in final edited form as: Cell Rep. 2019 Dec 3;29(10):3303–3312.e3. doi: 10.1016/j.celrep.2019.10.124

Figure 3. nBitbow Efficiently Identifies Neuronal Lineages of Drosophila PNS Neurons.

Figure 3.

(A) Schematic drawing of the distribution of PNS neurons in one hemisegment of the larval body wall. In the PNS, multipolar neurons include the md, da, bipolar dendrite (bd), and td types, whereas bipolar neurons include the es and chordotonal (ch) types.

(B) Schematic drawing of the experimental steps.

(C) Representative fluorescent images that show matching color codes of pairs of neurons previously thought to be independent of each other. These neuronal pairs are the five es neurons (desC, lesC, v’esA, vesA, and vesB) and their lineage-related da or td neurons. These es neurons have been previously reported as the only neuron from individual SOPs (Brewster and Bodmer, 1995). Larvae were heat-shocked at 3–4 h AEL. Scale bar: 10 μm.

(D) Statistical analysis confirms that these neuron pairs are lineage related. The κ values calculated based on experimental observations (red arrows) are significantly higher (i.e., closer to 1) than those calculated based on a random color distribution (blue).

See also Figures S1 and S2.