Table 3. Primer sequences.
Target gene | Primer sequences |
---|---|
Human USP6 F | 5′-TGAGCCCGTTGGAATCAACA-3′ |
Human USP6 R | 5′-ATCCACTTGCTCGTTCGTGT-3′ |
Human USP32 F | 5′-AGATCAGGCTCCGACCCC-3′ |
Human USP32 R | 5′-AGATCAGGCTCCGACCCC-3′ |
Human TBC1D3 F | 5′-AGTGGTCTACAGCAGTTACACA-3′ |
Human TBC1D3 R | 5′-GCTCGGTGTCCCTTTTCGTA-3′ |
Human β-actin F | 5′-CAACCGCGAGAAGATGAC-3′ |
Human β-actin R | 5′-GTCCATCACGATGCCAGT-3′ |
Mouse GluA1 F | 5′-AGGGATCGACATCCAGAGAG-3′ |
Mouse GluA1 R | 5′-TGCACATTTCCTGTCAAACC-3′ |
Mouse GluA2 F | 5′-CAGTTTCGCAGTCACCAATG-3′ |
Mouse GluA2 R | 5′-ACCCAAAAATCGCATAGACG-3′ |
Mouse GluN1 F | 5′-GGATACCAGATGTCCACCAGACTAAAG-3′ |
Mouse GluN1 R | 5′-AACGCAGAAGCCATAACAGCAC-3′ |
Mouse GluN2A F | 5′-CGGGTCTCATTTCAGTCTCTTACG-3′ |
Mouse GluN2A R | 5′-GGTTGTCATCTGGCTCACAGTCAG-3′ |
Mouse GluN2B F | 5′-GCGATTTGGTTACTCTGGGGTC-3′ |
Mouse GluN2B R | 5′-GTCTCTGGAACTTCTTGTCACTCAGG-3′ |
Mouse β-actin F | 5′-GCCAACCGTGAAAAGATGAC-3′ |
Mouse β-actin R | 5′-GAGGCATACAGGGACAGCAC-3′ |
Abbreviations: F, forward; Glu, glutamate ionotropic receptor; R, reverse; TBC, Tre-2/USP6, BUB2, and Cdc16; TBC1D3, TBC1 domain family member 3; USP, ubiquitin-specific protease