Figure 3: Loss of Efm methyltransferases results in slow growth in solid and liquid YPD growth media while EF1A with four lysine to arginine mutations shows slow growth in only liquid media.

A) Yeast cells from wildtype and mutant strains grown at 30 °C in YPD to an OD_{600 nm} of about 0.5 and 3 μl of a cell suspension starting at 0.1 OD_{600 nm} were then serially diluted and plated on YPD agar plates at 30 °C. Colonies were photographed for a representative experiment after 1 day or 2 days. In replicate experiments, we found that colonies for the efm1456Δ mutant were significantly smaller than wildtype colonies in 16 out of 23 experiments; in the other 7 cases colonies were roughly the same size. Colonies for the efm14567Δ mutant were significantly smaller than wildtype colonies in 19 out of 21 replicate experiments; in the other 2 cases colonies were roughly the same size. In 23 replicate experiments the colony sizes for the TEF1 K(30,79,316,390)R mutants were indistinguishable from the wildtype. B) Doubling times for growth in liquid YPD media at 30 °C were calculated from the linear portion of exponential growth measured by OD_{600 nm} over a 12 h time frame. Each point is a biological replicate. Error bars indicate standard deviation values and Student t-test p values (unpaired, two tails) are shown.