Figure 1. NOD.Il27^−/− Mice Are Completely Resistant to T1D.

(A) T1D incidence of NOD and NOD.Il27^−/− mice. ***p < 0.005 by log rank test.

(B) Summary of insulitis in female NOD and NOD.Il27^−/− mice.

(C) Summary of insulitis in male NOD and NOD.Il27^−/− mice. Pancreatic islets were scored for insulitis: 0 = no infiltration, 1 = peri-insulitis, 2 = ≤25% β cell loss, 3 = between 25% and 75% β cell loss, 4 = >75% β cell loss. Each symbol represents one mouse. The horizontal bar depicts the mean. More than 30 islets were scored for each mouse. **p < 0.01 by Mann-Whitney test. NS, not significant.

(D) T1D incidence study of sublethally irradiated NOD.Il27^−/− females infused with BM cells (5 × 10⁶) from sex-matched NOD.Rag1^−/− or NOD.Rag1^−/−.Il27^−/− mice as indicated. **p < 0.01 by log rank test.

(E and F) T1D incidence study of adoptively transferred T cell recipients.

(E) Splenic T cells (5 × 10⁶) were isolated from 6-week-old NOD females and transferred into sex-matched NOD.Rag1^−/− or NOD.Rag1^−/−.Il27^−/− recipients.

(F) Splenic T cells (5 × 10⁶) were isolated from young (6- to 7-week-old) NOD, old (11- to 13-week-old) NOD, or old (14- to 15-week-old) NOD.Il27^−/− mice and transferred into sex-matched NOD.Rag1^−/− recipients. *p < 0.05, **p < 0.01, and ***p < 0.005 by log rank test. NS, not significant.

See also Figure S1.