Table 1.
Lineage tracing for intramyocardial coronary VECs in embryonic ventricle wall
| Mouse line | Targeting strategy | Constitutive / inducible | Origins / sources | % VECs labeled | Note | References |
|---|---|---|---|---|---|---|
| WT280Cre, YAC | Transgenic | Constitutive | Proepicardium | 14% | Labels most epicardium | (Wilm et al. 2005) |
| Wt1/IRES/GFP-Cre, BAC | Transgenic (RP23-266M16) | Constitutive | Proepicardium | Few | Labels most epicardium | (Wessels et al. 2012) |
| Wt1(RP23–8C14)-Cre | Transgenic (RP23–8C14) | Constitutive | Proepicardium | Few | Labels most epicardium | (Kolander et al. 2014) |
| Wt1-GFPCre | Knockin (ATG) | Constitutive | Proepicardium | Few | Labels most epicardium; caution regarding non-epicardial labeling under some conditions. |
(Zhou et al. 2008, 2012; Rudat and Kispert 2012). |
| Wt1-CreERT2 | Knockin (ATG) | Inducible | Proepicardium | Few | Labels most epicardium; caution regarding non-epicardial labeling under some conditions. |
(Zhou et al. 2008, 2012) |
| Tbx18-Cre | Knockin (5’ UTR) | Constitutive | Proepicardium | None | Labels most epicardium | (Cai et al. 2009) |
| Tcf21 MerCreMer | Knockin (ATG) | Inducible | Epicardium | None | Labels most epicardium | (Asha et al. 2011) |
| Tbx18-CreERT2 | Knockin (ATG) | Inducible | Epicardium | None | Labels most epicardium | (Moore-Morris et al. 2014) |
| VE-Cad CreERT2 | Transgenic | Inducible | Sinus venosus | Most | Clonal analysis | (Red-Horse et al. 2010) |
| Scx-GFPCre, BAC | Transgenic | Constitutive | Proepicardium | ND* (1) | Labels part of SV | (Katz et al. 2012) |
| Sema3D-Cre | Knockin (ATG) | Constitutive | Proepicardium | ND* (2) | Labels part of SV | (Katz et al. 2012) |
| Nfatc1-IRES-Cre | Knockin (3’ UTR) | Constitutive | Endocardium | 81% | Labels part of SV | (Wu et al. 2012) |
| Nfatc1-nrtTA, BAC | Transgenic | Inducible | Endocardium | Most | Clonal analysis | (Wu et al. 2012) |
| Nfatc1-CreERT2 | Knockin (ATG) | Inducible | Endocardium | Few | Labels most 2nd CVP | (Tian et al. 2014) |
| Apj-CreERT2 | Transgenic | Inducible | Sinus venosus | 40% LV | Labels part of SV | (Chen et al. 2014) |
| Apln-CreERT2 | Knockin (ATG) | Inducible | Subepicardial VECs | >90% | Labels subepicardial vessels | (Tian et al. 2013) |
Few means the minority, as there is no quantitative data available from published papers.
Most means the majority of clones show their relationship by clonal analysis
ATG means the Cre or CreERT2 cDNA cassette was knocked into the endogenous start codon.
3’UTR means the Cre or CreERT2 cDNA cassette is inserted within the 3’ untranslated region as IRES following endogenous gene.
ND, not determined; LV, left ventricle.
*
(1) 24.3% or (2) 6.9% of Cre-labeled cells were coronary VECs.