Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Saccharomyces cerevisiae) | HRD1 | This study | YOL013C | Amplified from BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | HRD3 | This study | YLR207W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | USA1 | This study | YML029W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | DER1 | This study | YBR201W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | YUH1 | This study | YJR099W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP1 | This study | YDL122W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP2 | This study | YOR124C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP3 | This study | YER151C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP5 | This study | YER144C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP6 | This study | YFR010W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP7 | This study | YIL156W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP8 | This study | YMR223W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP9 | This study | YER098W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP10 | This study | YNL186W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP11 | This study | YKR098C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP12 | This study | YJL197W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP13 | This study | YBL067C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP14 | This study | YBR058C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP15 | This study | YMR304W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | UBP16 | This study | YPL072W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | DOA4 | This study | YDR069C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | SAD1 | This study | YFR005C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | OTU1 | This study | YFL044C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | OTU2 | This study | YHL013C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | RRI1 | This study | YDL216C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | RPN11 | This study | YFR004W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | RPN8 | This study | YOR261C | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | ORM2 | This study | YLR350W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | ERG3 | This study | YLR056W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | HMG2 | This study | YLR450W | BY4741 (genomic DNA) |
| Gene (S. cerevisiae) | PRC1 (CPY) | This study | YMR297W | BY4741 (genomic DNA) |
| Gene (Homo sapiens) | Ubiquitin specific protease 2; Usp2 | This study | AAP36388.1 | Synthetic gene |
| Strain, strain background (S. cerevisiae) | BY4741 | GE Dharmacon | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 | |
| Strain, strain background (S. cerevisiae) | hrd1Δ | GE Dharmacon | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 hrd1::kanR | |
| Strain, strain background (S. cerevisiae) | hrd3Δ | GE Dharmacon | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 hrd3::kanR | |
| Strain, strain background (S. cerevisiae) | usa1Δ | GE Dharmacon | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 usa1::kanR | |
| Strain, strain background (S. cerevisiae) | der1Δ | GE Dharmacon | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 der1::kanR | |
| Strain, strain background (S. cerevisiae) | ubp1Δ | This study | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 ubp1::kanR | |
| Strain, strain background (S. cerevisiae) | yRB057A | This study | MATa his3Δ1 leu2Δ0 MET15 lys2Δ0 ura3Δ0 hrd1::kanR hrd3::kanR | |
| Strain, strain background (S. cerevisiae) | yBGP15A | This study | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 hrd1::kanR ubp1::kanR | |
| Strain, strain background (S. cerevisiae) | yRB0065A | This study | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 hrd3::kanR usa1::kanR | |
| Strain, strain background (S. cerevisiae) | yRB0126 | This study | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 hrd3::kanR ubp1::hphNT1 | |
| Strain, strain background (S. cerevisiae) | yRB0075C | This study | MAT? his3Δ1 leu2Δ0 MET15 ura3Δ0 hrd3::kanR usa1::kanR ubp1::hphNT1 | |
| Genetic reagent (S. cerevisiae) | CPY* | (Finger et al., 1993) | G255R point mutation in PRC1 (CPY) leading to CPY* | |
| Genetic reagent (S. cerevisiae) | GPD promoter | This study | YGR192C | 667 basepairs upstream of TDH3 (GPD) gene; BY4741 (genomic DNA) |
| Antibody | THE Anti-DYKDDDK Antibody (mAb, mouse) | Genscript | A00187 | 1:2000 (5% milk in TBST) |
| Antibody | Anti-HA High Affinity Antibody (mAb, clone 3F10, rat) | Roche | 11867423001 | 1:2000 (5% milk in TBST) |
| Antibody | THE V5 Tag Antibody(mAb, mouse) | Genscript | A01724 | 1:2500 (5% milk in TBST) |
| Antibody | Anti-PGK1 (mAb, clone22C5D8, mouse) | Invitrogen | 459250 | 1:1000 (5% milk in TBST) |
| Antibody | Anti-Ubiquitin(mAb, clone P4D1, mouse) | Santa Cruz Biotechnologies | sc-8017 | 1:500 (5% milk in TBST) |
| Antibody | Amersham ECL Rabbit IgG, HRP-linked wholeAb (donkey) | GE Healthcare | NA934 | 1:4000 (5% milk in TBST) |
| Antibody | Amersham ECL Mouse IgG, HRP-linked whole Ab (sheep) | GE Healthcare | NA931 | 1:4000 (5% milk in TBST) |
| Antibody | Amersham ECL Rat IgG, HRP-linked whole antibody (goat) | GE Healthcare | NA935 | 1:4000 (5% milk in TBST) |
| Commercial assay or kit | Western Lightning Plus-ECL, Enhanced Chemiluminescence Substrate | Perkin-Elmer | NEL103E001 | |
| Commercial assay or kit | Amersham ECL Select Western Blotting Detection Reagent | GE Healthcare | RPN2235 | |
| Chemical compound, drug | SYTOX Blue Nucleic Acid Stain | Invitrogen | S11348 | |
| Chemical compound, drug | Cycloheximide | Calbiochem | 239763 | |
| Chemical compound, drug | Decyl Maltose Neopentyl Glycol (DMNG) | Anatrace | NG322 | |
| Chemical compound, drug | Zaragozic Acid A | Cayman Chemical | 17452 | |
| Software, algorithm | Graphpad Prism 8 | Graphpad Software LLC. | version 8.3.0 | |
| Software, algorithm | FlowJo | Becton, Dickinson and Company | Version 10.6.0 | |
| Other | Dynabeads His-Tag Isolation and Pulldown | Invitrogen | 10104D | |
| Other | Anti-V5 Agarose Affinity Gel | Millipore | A7345 |