Video 1. Autophagy flux in cells expressing an empty (control) vector.

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HeLa cells stably expressing mRFP-GFP-LC3 were transfected with empty vector (Video 1) or TBC1D15 overexpression vector (Video 2) and live cell imaging was conducted the following day. Notice the presence of large immobile mRFP- and GFP-positive autophagosomes in TBC1D15 overexpressing cells, implying a block in autophagosome maturation. Cells were imaged once every second for a period of 2 min and the movies are displayed at a speed of 10 frames per second.