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. 2019 Nov 27;11(22):10723–10741. doi: 10.18632/aging.102517

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Copyright © 2019 Alberro et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Plasma extracellular vesicle characterization. T cell and senescence markers are present on plasma EVs, but no differences were found between EVs from different age ranges (n=49 donors). (A) Representative cryoEM image of isolated EVs. (B) Representative figure of particle size distribution of EVs obtained by NTA. (C) Percentage of particles positive for EV characteristic markers assessed by flow cytometry. (D, E) Senescence markers on CD3+CD4+ and (F-G) on CD3+CD8+ EVs assessed by flow cytometry. Age range in years.