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. 2019 Dec 11;12:10851–10861. doi: 10.2147/OTT.S220072

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© 2019 Wang et al.

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miR-124-3p is bound to HOXA-AS2. (A) The potential binding sites of HOXA-AS2 and miR-124-3p. (B and C) Luciferase activity was measured in U2OS and MG-63 cells co-transfected with miR-124-3p or miR-NC and HOXA-AS2 WT or HOXA-AS2 MUT. (D) The enrichment of HOXA-AS2 was measured in the two cells transfected with miR-124-3p or miR-NC after Ago2 RIP. (E and F) The level of miR-124-3p was detected in U2OS and MG-63 cells after RNA pull-down. (G and H) The expression of miR-124-3p was detected in osteosarcoma tissues and cells by qRT-PCR. (I) The association between HOXA-AS2 and miR-124-3p levels was analyzed in osteosarcoma patients. (J) The level of miR-124-3p was measured in U2OS and MG-63 cells transfected with si-NC or si-HOXA-AS2 by qRT-PCR. *P<0.05.