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. 2019 Dec 10;10:2836. doi: 10.3389/fmicb.2019.02836

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Copyright © 2019 Han, Ao, Lin, Guan, Zheng, Han and Ye.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Workflow of TMT quantitatively proteomics analysis. A total of 54 serum samples from dengue haemorrhagic fever (DHF), Dengue fever (DF), and healthy groups were collected, numbered A, B, and C, with 18 in each group. After protein extraction, the serum proteins of every six people in the group were mixed into a mixed pool for TMT labeling. The order of labeling is: A1-126, A2-127N, A3-127C, B1-128C, B2-129N, B3-129C, C1-130N, C2-130C, C3-131. When the labeling was completed, all the proteins were mixed and rotated to dry. The 1D LC separation – high-pH reverse-phase separation was performed after resolving. The separated components are combined and evaporated to dryness and then re-dissolved. LC-MS/MS analysis was performed after that.