Table 1.

Determination of intra-assay precision for TMUV PRNT.

Sample no.	ND50 for antibody-negative sera†			Sample no.	ND50 for antibody-negative sera‡			Sample no.	ND50 for anti-Y4 sera
	Test 1	Test 2	Test 3		Test 1	Test 2	Test 3		Test 1	Test 2	Test 3
1	10	16	10	1	13	10	10	1	12,589	15,848	7,943
2	13	20	20	2	25	16	20	2	6,309	10,000	7,943
3	50	63	40	3	25	13	32	3	50,118	31,622	39,811
4	13	16	12	4	32	40	40	4	7,943	10,000	12,589
5	63	79	50	5	126	100	63	5	31,622*	10,000	10,000
6	32	25	40	6	100	79	79	6	5,012	6,309	10,000
7	126	79	63	7	100	79	25*	7	15,849	19,953	15,849
8	100	126	79	8	63	79	50	8	25,119	25,119	39,811
9	32	50	63	9	100	100	126	9	63,096	79,433	19,953*
10	13	16	10	10	40	32	32	10	10,000	12,589	15,849

^†Sera collected from 1-day-old ducklings.

^‡Sera prepared from 14-days-old ducklings that had been inoculated with DMEM at 7 days of age.

^*Outside ± 3-fold of the median titer of three results obtained in triplicates.