Table 3.

Evaluation of the sensitivity for TMUV PRNT.

Sample no.	ND50 titer of neutralizing antibody in serum sample with dilution indicated
	Undiluted	1:10		1:100		1:1,000
	Median†	Median	Expected‡	Median	Expected	Median	Expected
1	39,811	5,012	3,981	794	398	20	40
2	7,943	1,000	794	50	79	16	8
3	15,849	3,162	1,585	79	159	13	16
4	15,849	1,995	1,585	100	159	50	16
5	50,119	6,310	5,012	316	501	63	50
6	15,848	1,000	1,585	200	159	20	16
7	12,589	1,000	1,259	79	126	32	13
8	31,622	5,012	3,162	200	316	63	32
9	12,589	2,511	1,259	126	126	25	13
10	79,433	7,943	7,943	501	794	125	80

^†Median titer, mean value of three titers obtained in triplicates from each of diluted serum samples.

^‡Expected titer, median titer of the undiluted sample divided by the dilution factor.