Table 2.
Antibodies used in this study.
| Antigen | Host | Manufacturer | Cat. No. | Application | Dilution |
|---|---|---|---|---|---|
| NES1 | Rabbit | Phoenix | H-003-22 | WB | 1:1,000 |
| IHC | 1:2,000 | ||||
| IF | 1:500 | ||||
| POMC | Mouse | Abnoa | MAB11358 | WB | 1:1,000 |
| IHC | 1:400 | ||||
| IF | 1:200 | ||||
| OT | Mouse | Merck | MAB5296 | IHC | 1:5,000 |
| IF | 1:600 | ||||
| MC4R | Rabbit | Abcam | Ab24233 | WB | 1:300 |
| GAPDH | Rabbit | TDYbio | TDY052F | WB | 1:5,000 |
| Alexa Fluor® 488 | Goat | Abcam | ab150077 | IF | 1:200 |
| Alexa Fluor® 594 | Goat | Abcam | ab150116 | IF | 1:200 |
| Goat anti-mouse IgG (H + L) | Goat | ZSGB-BIO | ZB-2305 | WB | 1:5,000 |
| Goat anti-rabbit IgG (H + L) | Goat | ZSGB-BIO | ZB-2010 | WB | 1:7,000 |