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. 2019 Dec 10;10:1477. doi: 10.3389/fphar.2019.01477

Figure 3.

Figure 3

Effect of catalpol on podocyte injury in DN mice. (A) Representative transmission electron microscopy (TEM) images of podocyte foot processes in Control, DN, and DN plus catalpol-treated (30, 60, and 120 mg/kg, weeks 1–8) mice (n = 3). Scale bar: 1 µm. Representative images of glomeruli showing nephrin-positive and synaptopodin-positive expression in glomeruli of Control, DN, and DN plus different doses of catalpol-treated mice (n = 8). Scale bar: 10 µm. (B) Quantification of podocyte foot process width by electron micrographs (n = 3, 10 images from each group). (C) Quantification of results for immunohistochemical staining of nephrin expressed as the percentage of stained area in glomeruli (n = 8, 20–25 images from each group). (D) Quantification of results in synaptopodin in glomeruli by the ratio of integrated optical density (IOD) to area (n = 8, 21–25 images from each group). (E) Western blotting for expression of synaptopodin in Control, DN, and DN plus different doses of catalpol-treated mice (n = 3). (F) Quantification of results in panel E. Data represent the mean values ± SD. **p < 0.01 vs Control, # p < 0.05, ## p < 0.01 vs DN.