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. 2019 Dec 10;10:1584. doi: 10.3389/fpls.2019.01584

Figure 5.

Figure 5

Proline accumulation and expression of the P5CS1 and PDH1 genes in salt-treated wild-type and hy5hyh double mutant plants. (A) Experimental design: 14-day-old in vitro-grown plants were conditioned by dark treatment for 5 days and subsequently treated with or without 150 mM NaCl and illuminated with white or monochromatic red or blue light. (B) Proline accumulation in Wassilewskija (WS) wild-type and hy5hyh mutant plants after 3 days of salt treatment. Error bars indicate SD (N = 5). (C, D) Transcript levels of the P5CS1(C) and PDH1(D) genes after 6 and 24 h of salt treatment. Relative transcript levels are shown, which were normalized to ACT2 and UBQ1 reference genes as well as to dark-conditioned plants. Abbreviations: Ctr: control, NaCl: salt treatment. Error bars indicate SD (N = 3). Significant differences between wild type and mutant values are: * p < 0.05, ** p < 0.01 (two-way ANOVA, Tukey test, fixed parameters were genotypes and treatments).