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Downregulation of HAVCR1 impaired the proliferation and colony formation of GAC cells. (a and b) A CCK-8 assay was used to measure the proliferation of MKN-45 and AGS cells. (c and d) A colony formation assay was conducted in MKN-45 and AGS cells to further determine the impact of HAVCR1 deficiency on clonogenic capability. And the number of clones was counted. ∗∗p < 0.01 versus the si-con group.
