Figure 3.
Effect of FA-97 on H2O2-induced oxidative stress in SH-SY5Y and PC12 cells. SH-SY5Y and PC12 cells were treated with H2O2 (500 μM) and FA-97 (0, 0.25, 0.5, and 1 μM) for 24 h. (a) Representative images of SH-SY5Y and PC12 cells stained with DCFH-DA (a ROS fluorescence probe). (b–e) The malondialdehyde (MDA) level (b), glutathione (GSH) level (c), superoxide dismutase (SOD) activity (d), and protein carbonylation (e) were measured according to the kit manufacturer's instructions. Scale bars, 200 μm. Results are representative of three independent experiments and expressed as means ± SD. #P < 0.05 and ##P < 0.01 compared with the control group and ∗P < 0.05 and ∗∗P < 0.01 compared with the H2O2-stimulated group.