Figure 4.
Effect of FA-97 on Nrf2/HO-1 signaling in SH-SY5Y and PC12 cells. SH-SY5Y and PC12 cells were treated with H2O2 (500 μM) and FA-97 (0, 0.25, 0.5, and 1 μM) for 24 h. (a) The expression of HO-1, NQO-1, Nrf2, and β-actin was detected by Western blot. (b) After being transfected with ARE-luciferase reporter plasmid, the Nrf2 transcription activity in SH-SY5Y and PC12 cells was detected by luciferase activity assay. (c) The expressions of Nrf2 in cytosolic and nuclear extracts were determined by Western blot. Lamin A/C and GAPDH were used as nuclear and cytoplasmic markers, respectively. (d, e) SH-SY5Y cell slides (d) and PC12 cell slides (e) were immune-stained with anti-Nrf2 (green) and DAPI (blue), and then the nuclear translocation of Nrf2 was observed by confocal laser-scanning microscope. Scale bars, 15 μm. The results are representative of three independent experiments and expressed as means ± SD. ##P < 0.01 compared with the control group and ∗P < 0.05 and ∗∗P < 0.01 compared with the H2O2-stimulated group.