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. 2019 Dec 3;2019:8239642. doi: 10.1155/2019/8239642

Figure 8.

Figure 8

Effect of FA-97 on scopolamine-induced oxidative stress and the Nrf2 pathway in vivo. Brain tissues were homogenized in cold PBS. (a, b) The levels of MDA (a) and total antioxidant capacity (b) in the hippocampus and cortex were measured according to the kit manufacturer's instructions. (c) The expressions of HO-1, NQO-1, and β-actin in the hippocampus and cortex were measured by Western blot analysis. (d) The nuclear Nrf2 expressions in the hippocampus and cortex were detected. The relative expressions of HO-1, NQO-1 (e), and Nrf2/Lamin A (f) were represented by densitometric analysis. (g) The expressions of HO-1 and NQO-1 in brain sections were detected by immunohistochemistry (IHC), and the positive cells were brown. (h) Quantification of IHC images was by Image-Pro Plus software, and 10 fields were counted for each mouse. IOD of HO-1- and NQO-1-positive cells were shown. The results are representative of three independent experiments and expressed as means ± SD. ##P < 0.01 compared with the control group; ∗P < 0.05 and ∗∗P < 0.01 compared with the SCOP-treated group.