Figure 2.

Ectopic NANOG expression and PBX1 overexpression promote proliferation and regulate the cell cycle of hair follicle-derived mesenchymal stem cells (HF-MSCs). (a, b) Images and Western blotting showed ectopic NANOG expression or PBX1 overexpression after lentiviral transduction. Scale bar = 200 μm. (c) Growth curves showed that NANOG and PBX1 promoted proliferation in comparison to that of control (vector only) cells. (d–e) Cell cycle was measured by flow cytometry, which revealed an effect on cell cycle phase distribution. The proliferation indexes of NANOG- and PBX1-expressing cells were higher than that of the control (vector; ^∗P < 0.05, ^∗∗P < 0.01).