Table 2.
Statistic data of concentration curves of IA in the time range of 0–30 min.
Time (min) | K m (mmol/l) | V max (nkat) | R | k cat (s−1) | LOD (mmol/l) |
---|---|---|---|---|---|
10 | 13.6 | 95.1 | 0.998 | 0.183 | 3.67 |
15 | 12.3 | 84.7 | 0.999 | 0.163 | 3.15 |
20 | 11.2 | 74.0 | 0.999 | 0.142 | 3.00 |
25 | 10.4 | 65.7 | 0.999 | 0.126 | 2.89 |
30 | 9.85 | 59.1 | 0.999 | 0.114 | 2.78 |
K m—Michaelis–Menten constant, Vmax—maximum velocity, R—correlation coefficient, kcat—turnover number, LOD—limit of detection. The values belong to the volume 5 μl of lipase (100 U).