Table 3.
Time (min) | K m (mmol/l) | V max (nkat) | R | k cat (s−1) | LOD (mmol/l) |
---|---|---|---|---|---|
10 | 9.27 | 163 | 0.999 | 0.157 | 0.209 |
15 | 9.16 | 133 | 0.999 | 0.127 | 0.122 |
20 | 8.72 | 113 | 0.999 | 0.108 | 0.102 |
25 | 8.12 | 96.5 | 0.999 | 0.093 | 0.100 |
30 | 8.49 | 90.3 | 0.999 | 0.087 | 0.092 |
K m—Michaelis–Menten constant, Vmax—maximum velocity, R—correlation coefficient, kcat—turnover number, LOD—limit of detection. The values belong to the volume 10 μl of lipase (200 U).