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. 2019 Dec 1;2019:7198215. doi: 10.1155/2019/7198215

Figure 1.

Figure 1

Initial characterization of UC-MSC according to three isolation methodologies. (a) Percentage of positive umbilical cords (UC) processed by each isolation method (n = 10‐12 donors per group). (b) Time of cell derivation (P0 to P1) for every isolation method (n = 10‐12 donors per group). (c) Population doubling time measured in early cell passages (P2 to P6) per isolation method (n = 10 donors per group). (d) Flow cytometry analyses of MSC identity markers (CD90, CD73, CD105, HLA-AB, HLA-DR, CD45, and CD34) as shown in total frequency. Level of expression for CD90, CD73, CD105, and HLA-Ab is presented as Median Fluorescence Intensity (MFI) (n = 4 donors per group). ∗ indicates p < 0.05, as evaluated by ANOVA.