Table 2.

Clones of 4T1luc2 expressing consensus RT of HIV-1 clade A FSU_A strain (RT_A) and its variants with primary mutations of resistance to NRTI (RT_An) and NNRTI (RT_Ann), obtained by lentiviral transduction of 4T1luc2 cells at different multiplicities of infection (MOI).

Enzyme	Polymerization Vmax∗, relative to RT_A∗∗	RNase H activity Vmax∗, relative to RT_A∗∗	Lentivirus	MOI	Derivative clone
RT-A	100%	100%	LV-RT-A	1	4T1luc2_RT-1.3
			LV-RT-A	5	4T1luc2_RT-5.3
			LV-RT-A	20	4T1luc2_RT-20.1
RT-An	59%	107%	LV-RT-An	1	4T1luc2_RT-An-1.4
			LV-RT-An	10	4T1luc2_RT-An-10.1
RT-Ann	81%	62%	LV-RT-Ann	1	4T1luc2_RT-Ann-1.5
			LV-RT-Ann	10	4T1luc2_RT-Ann-10.2

^∗ V _max for polymerase and RNase H activities according to Table 1. ^∗∗Mean V_max for the given RT_A variant divided by mean V_max for RT_A, in %.