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. 2019 Dec 1;2019:7606238. doi: 10.1155/2019/7606238

Figure 6.

Figure 6

Effects of PAME on p53 protein stability. PAME significantly increased the (a) mRNA level of p21 but not p53 (n = 5) and reduced the (b) level of Mdm2 protein (n = 10). The mRNA levels were detected by qRT-PCR, and the protein level was determined by Western blot analysis. (c) PAME prolonged the degradation of p53 protein in hBM-MSCs. The dotted line indicates 50% abundance of p53, and the half-life (t1/2) of p53 protein in each group is shown in the bottom panel. hBM-MSCs were treated with PAME (50 μM) for 6 h followed by CHX (5 μM) treatment and then subjected to protein level detection by Western blot analysis (n = 4-6). All data represent mean ± SEM. ∗p < 0.05, versus the control group. Con: control; CHX: cycloheximide.