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Morphology and identification of BMSCs. (a) Morphology of the BMSCs. Phenotypic characterization of the cultured BMSCs. (b) Immunofluorescent staining of BMSC surface markers CD29, CD34−, and CD45−. (c) Flow cytometry analysis of the cell surface markers CD45, CD29, and CD90 on cultured BMSCs at the third passage. (d) Flow cytometry analysis of VLA4 expression on cultured BMSCs at the third passage.
