Table I:
particulate | Amount (mg) | % of microsomes bounda |
---|---|---|
anthophyllite | 4.2 | 44 |
anthophyllite | 8.3 | 73 |
anthophyllite | 16.7 | 91 |
anthophyllite | 20.0 | 96 |
anthophyllite | 33.4 | 91 |
iron oxide | 4.2 | 38 |
iron oxide | 8.3 | 63 |
iron oxide | 16.7 | 83 |
iron oxide | 20.0 | 87 |
iron oxide | 33.4 | 89 |
silica | 16.7 | 40 |
Canadian chrysotile | 16.7 | 84 |
carbon black | 16.7 | 53 |
Binding of microsomes to particulates was quantitated by incubation of microsomes (equivalent to 0.6 mg of microsomal protein) with the amount of particles shown for 30 min at 25 °C with continual stirring. The 480g supernatant was then assayed for protein to determine the extent of binding.