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. 2019 Dec 3;7:371. doi: 10.3389/fbioe.2019.00371

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Copyright © 2019 do Amaral, Zayed, Pascu, Cavanagh, Hobbs, Santarella, Simpson, Murphy, Sridharan, González-Vázquez, O'Sullivan, O'Brien and Kearney.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Platelet-rich plasma (PRP) was successfully incorporated into collagen-GAG scaffolds. Collagen-GAG scaffolds (A,D,G,J), PRP gel (B,E,H,K) and the composite scaffold (C,F,I,L) were visualized by naked eye (A–C), histology (D–F), immunofluorescence (G–I), and scanning electron microscopy (J–L). PRP-derived fibrin was evenly incorporated within the pores of collagen-GAG scaffolds. Aniline blue (blue) evidencing collagen (D,F) and Biebrich Scarlet-Acid Fucshin (red) evidencing fibrin (E,F). Collagen-GAG strut autofluoresced in blue (G,I) and fibrinogen immunostating in green (H,I). Star indicates the collagen-GAG strut in (J,L), and arrows indicates the fibrin network in (K,L).