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. 2019 Dec 3;7:371. doi: 10.3389/fbioe.2019.00371

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Copyright © 2019 do Amaral, Zayed, Pascu, Cavanagh, Hobbs, Santarella, Simpson, Murphy, Sridharan, González-Vázquez, O'Sullivan, O'Brien and Kearney.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tubule formation with platelet-rich plasma releasate (PRPr) and composite scaffold-conditioned medium. Representative images of HUVEC cultured after 7 and 48 h in growth factor-depleted Matrigel to induce tubulogenesis in the presence of basal (EBM) or growth (EGM) medium supplemented with FBS or different PRPr concentrations, as well as with a 24 h conditioned medium incubated with the composite scaffold. After 48 h in EBM it is possible to notice increased tubulogenesis in 2% PRPr and the composite scaffold compared to 2% FBS control. One hundred micrometer scale bars for 7 h pictures, and 200 μm scale bars for 48 h pictures.