Table 1.
Composition of experimental diets (as-fed basis)1
| Item | Phase 1 | Phase 2 |
|---|---|---|
| Ingredient, % | ||
| Corn, yellow | 42.96 | 54.58 |
| Soybean meal, 48% CP | 19.00 | 23.50 |
| Whey permeate | 20.00 | 10.00 |
| Blood plasma | 7.50 | 4.00 |
| Poultry meal | 3.20 | 2.00 |
| Fish meal | 2.00 | 0.00 |
| L-Lys HCl | 0.37 | 0.41 |
| DL-Met | 0.17 | 0.15 |
| L-Thr | 0.10 | 0.11 |
| Salt | 0.22 | 0.22 |
| Vitamin permix2 | 0.03 | 0.03 |
| Trace mineral permix3 | 0.15 | 0.15 |
| Dicalcium phosphate | 0.30 | 0.90 |
| Limestone | 1.00 | 0.95 |
| Poultry fat | 2.00 | 2.00 |
| Calculated composition | ||
| DM, % | 90.60 | 90.03 |
| ME, kcal/kg | 3,398 | 3,371 |
| CP, % | 23.40 | 21.52 |
| SID4 Lys, % | 1.50 | 1.35 |
| SID Cys + Met, % | 0.82 | 0.73 |
| SID Trp, % | 0.26 | 0.23 |
| SID Thr, % | 0.88 | 0.79 |
| Ca, % | 0.85 | 0.80 |
| STTD5 P, % | 0.44 | 0.40 |
| Total P, % | 0.64 | 0.63 |
| Nucleotides, mg/kg6 | 112.4 | 48.6 |
1Diets in each phase supplemented with increasing supplementation of 0, 0.005, 0.015, 0.025, and 0.05% nucleotides extracted from yeast cell (YT500, Hinabiotech, Guangzhou, China) for a total of 5 diets per phase.
2The vitamin premix provided per kilogram of complete diet: 6,614 IU of vitamin A as vitamin A acetate, 992 IU of vitamin D3, 19.8 IU of vitamin E, 2.64 mg of vitamin K as menadione sodium bisulfate, 0.03 mg of vitamin B12, 4.63 mg of riboflavin, 18.52 mg of D-pantothenic acid as calcium panthonate, 24.96 mg of niacin, and 0.07 mg of biotin.
3The trace mineral premix provided per kilogram of complete diet: 33 mg of Mn as manganous oxide, 110 mg of Fe as ferrous sulfate, 110 mg of Zn as zinc sulfate, 16.5 mg of Cu as copper sulfate, 0.30 mg of I as ethylenediamine dihydroiodide, and 0.30 mg of Se as sodium selenite.
4SID, standardized ileal digestible.
5STTD P, standardized total tract digestible phosphorus.
6Data from Mateo (2005) and Suresh et al. (2011).