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RBCs from three different volunteers were isolated and incubated in PBS containing 5 mM glucose, 100 μM ascorbate and with (black square) or without (grey square) ascorbate oxidase. After incubation for 2 h, cells were washed and transferred into PBS without vitamin C. Extracellular vitamin C accumulation (disposal from RBCs) was determined by HPLC analysis. Significant differences (P < 0.05) of ascorbate disposal are marked by asterisks
