Figure 1.

Assimilation Pattern of Different Nitrogen Sources

(A–D) Heatmaps are shown for amino acids derived from Mtb-infected macrophages (A), uninfected macrophages (B), intracellular Mtb (C), and in-vitro-grown Mtb in rosins minimal media (D). Assimilation patterns are shown for six tracers: ¹⁵N₁-Asp, ¹⁵N₁-Glu, ¹⁵N₂-Gln, ¹⁵N₁-Leu, ¹⁵N₁-Ala, and ¹⁵N₁ Gly. Essential amino acids for macrophages are highlighted in blue. Heatmaps were produced using enrichment (%) of amino acids recorded for each of the individual tracer experiments in Data S1. The maximum and minimum enrichments for each heatmap are described using color keys. Enrichments are shown for the following amino acids: alanine (Ala), m/z 260; glycine (Gly), m/z 246; valine (Val), m/z 288; leucine (Leu), m/z 274; isoleucine (Ile), m/z 274; methionine (Met), m/z 320; serine (Ser), m/z 390; threonine (Thr), m/z 404; proline (Pro), m/z 258; phenylalanine (Phe), m/z 336; aspartate/asparagine (Asp/n), m/z 418; glutamate/glutamine (Glu/n), m/z 432; lysine (Lys), m/z 431; arginine (Arg), m/z 442; and tyrosine (Tyr), m/z 466, measured using GC-MS.