Figure 2.

Nitrogen Assimilation is Compartmentalized in Mtb

(A) Distinct enrichment of amino acids from each of the tracers in intracellular Mtb.

(B) ¹⁵N assimilation from the tracers into the amino acids in in-vitro-grown Mtb.

(C) Comparison of ¹⁵N enrichment from the tracers ¹⁵N₁-Glu and ¹⁵N₂-Gln in an infected macrophage.

(D) Comparison of ¹⁵N enrichment from the tracers ¹⁵N₁-Glu and ¹⁵N₂-Gln in intracellular Mtb.

Values are mean ± SEM from 3–4 biological replicates. Statistically significant differences were calculated using Holm-Sidak multiple t tests; ^∗p < 0.001.