REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit polyclonal anti-Rps4x	Proteintech	Cat#14799-1-AP; RRID: AB_2238567
Rabbit polyclonal anti-Rpl17	Proteintech	Cat#14121-1-AP; RRID: AB_2253985
Rabbit polyconal anti-Rps14	Abcam	Cat#ab174661
Mouse monoclonal anti-Rpl19	Abcam	Cat#ab58328; RRID: AB_945305
Mouse monoclonal anti-Rps23	Abcam	Cat#ab57644; RRID: AB_945314
Rabbit polyclonal anti-Rps3a	Proteintech	Cat#14123-1-AP; RRID: AB_2253921
Mouse monoclonal anti-Neurofilament A	DSHB	Cat#3A10; RRID: AB_531874
Rabbit polyclonal anti-Rpl39	Abcam	Cat#ab74740; RRID: AB_1524345)
Rabbit polyclonal anti-Rps12	Proteintech	Cat#16490-1-AP; RRID: AB_2146233
Rabbit monoclonal anti-Gtpbp4	Abcam	Cat#ab92342; RRID: AB_2049721
Mouse monoclonal anti-Npm1	Origene	Cat#BM5524; RRID: AB_1008764
Rabbit polyclonal anti-Abce1	Abcam	Cat#ab32270; RRID: AB_722514
Rabbit polyclonal anti-β-actin	Abcam	Cat#ab8227; RRID: AB_2305186
Rabbit polyclonal anti-β-catenin	Sigma-Aldrich	Cat#C2206; RRID: AB_476831
Mouse monoclonal anti-puromycin, clone 12D10	Sigma-Aldrich	Cat#MABE343; RRID: AB_2566826
Mouse monoclonal anti-ribosomal RNA (Y10B)	Abcam	Cat#ab171119
Rabbit monoclonal anti-biotin	Cell Signaling	Cat#5597; RRID: AB_10828011
Goat anti-rabbit Alexa Fluor 568	Abcam	Cat#ab150117; RRID: AB_2688012
Goat anti-mouse Alexa Fluor 594	Abcam	Cat#ab150080; RRID: AB_2650602
Mouse monoclonal anti-puromycin, clone 12D10, Alexa Fluor 647 Conjugate	Sigma-Aldrich	Cat#MABE343-AF647
Goat anti-mouse HRP	Abcam	Cat#ab6789; RRID: AB_955439
Goat anti-rabbit HRP	Abcam	Cat#ab97080; RRID: AB_10679808
Bacterial and Virus Strains
BioBlue Chemically Competent Cells	Bioline	Cat#BIO-85037
Chemicals, Peptides, and Recombinant Proteins
AHA (L-Azidohomoalanine)	ThermoFisher	Cat#C10102
Leibovitz’s L-15 Medium	ThermoFisher	Cat#11415064
Leibovitz’s L-15 medium -Lys -Arg	GIBCO Life technologies	N/A (customized)
Stable isotope-coded amino acids Lys8	Silantes GmbH	Cat#211603902
Stable isotope-coded amino acids Arg10	Silantes GmbH	Cat#20160390
Antibiotic-Antimycotic (100X)	Thermo Fisher Scientific	Cat#15240062
Poly-L-lysine	Sigma-Aldrich	Cat#P1274
Laminin	Sigma-Aldrich	Cat#L2020
Puromycin	Sigma-Aldrich	Cat#P8833
Cycloheximide	Sigma-Aldrich	Cat#C4859
Anisomycin	Sigma-Aldrich	Cat#A9789
Cy5-UTP	PerkinElmer	Cat#NEL582001EA
SUPERase In RNase Inhibitor	Ambion	Cat#AM2696
FluorSave	Merck Millipore (Calbiochem)	Cat#345789-20
Duolink In Situ Mounting Medium with DAPI	Sigma-Aldrich	Cat#DUO82040-5ML
Recombinant mouse Netrin-1	R&D systems	Cat#1109-N1
RNase A	Ambion	Cat#EN0531
Rnase T1	Ambion	Cat#EN0541
n-Octylglucoside	Sigma-Aldrich	Cat#10634425001
Critical Commercial Assays
NeuroPORTER transfection reagent	Sigma-Aldrich	Cat#NPT01
Rneasy mini kit	QIAGEN	Cat#74104
SuperScript II First Strand Synthesis kit	Thermo Fisher Scientific	Cat#18080051
Quantitect SYBR Green PCR kit	QIAGEN	Cat#204141
KAPA HyperPrep kit	Roche	Cat#KK8503
NextSeq 500/550 high output v2 kit (150 cycles)	Illumina	Cat#FC-404-2002
Duolink In Situ Detection Reagents Green	Sigma-Aldrich	Cat#DUO92014
Duolink In Situ PLA probe Anti-Rabbit PLUS	Sigma-Aldrich	Cat#DUO92002
Duolink In Situ PLA probe Anti-Mouse MINUS	Sigma-Aldrich	Cat#DUO92004
Click-iT Cell Reaction Buffer Kit	Thermo Fisher Scientific	Cat#10269
Deposited Data
Proteomics data	This study	PRIDE: PXD011032
		PRIDE: PXD015574
RNA-sequencing data	This study	GSE135502
Experimental Models: Organisms/Strains
Xenopus laevis	Nasco	Cat#LM00715, Cat#LM00535
Oligonucleotides
Morpholino: Control MO 5′-CCTCTTACCTCAGTTA CAATTTATA-3′	Gene tools	N/A
Morpholino: Rps4x.S MO 5′-TTCTTCGGTCCGCGA GCCATG-3′	Gene tools	N/A
Morpholino: Rps4x.L MO 5′-CTTTTTCGGTCCACG AGCCATTTTC-3′	Gene tools	N/A
Morpholino: Rpl5 MO 5′- ACCTTTACGAACCCC ATTTTGCTCT −3′	Gene tools	N/A
Morpholino: Rps3a.S MO 5′- TCTTGTTCTTGCCG ACTGCCATC −3′	Gene tools	N/A
Morpholino: Rps3a.S MO 5′- GTTCTTGCCCACTGC CATCTTGC −3′	Gene tools	N/A
Primers for 18S rRNA (Xenopus laevis) qRT-PCR forward: 5′-GTAACCCGCTGAACCCCGTT-3′	This study	N/A
Primers for 18S rRNA (Xenopus laevis) qRT-PCR reverse: 5′-CCATCCAATCGGTAGTAGCG-3′	This study	N/A
5′RACE oligo 1: 5′-GGTCCACGAGCAAAGACA CCAGTCAA −3′	This study	N/A
5′RACE oligo 1st PCR: 5′- GCAAAGACACCAGTCA ACTTGTCCAACATC −3′	This study	N/A
5′RACE oligo 2nd PCR: 5′- AACACGCTTCAAGTGCT TTTTCGGTCCA −3′	This study	N/A
5′RACE sequencing oligo: 5′- GTGCTTTTTCGGT CCACGAGCAAAGAC −3′	This study	N/A
Primers for 18S pre-rRNA (Xenopus laevis) RT-PCR forward: 5′-GAGCGAGAGAGAAAGACGGA −3′	This study	N/A
Primers for 18S pre-rRNA (Xenopus laevis) RT-PCR reverse: 5′-TCTAGAGTCACCAAAGCGGC −3′	This study	N/A
Recombinant DNA
Plasmid: pCS2+-Venus-Rps4x-Full length	This study	N/A
Plasmid: pCS2+-Venus-Rps4x-Del CUIC	This study	N/A
Plasmid: pCS2+-Venus-Rps4x-Del 5′UTR	This study	N/A
Plasmid: pCS2+-Venus-Rps4x-Del 5′UTR-3′UTR	This study	N/A
Plasmid: pCS2+-Venus-Rps4x (Xenopus)	This study	N/A
Plasmid: pCS2+-mGFP	Das et al., 2003	N/A
Plasmid: pCS2+-mRFP	Poggi et al., 2005	N/A
Plasmid: pCS2+-Mo resistant Rps4x del-5’UTR/mGFP dual promotor	This study	N/A
Plasmid: pCS2+-Mo resistant Rps4x del-CUIC/mGFP dual promotor construct	This study	N/A
Plasmid: pCS2+-MO resistant Rps4x/mGFP dual promotor construct	This study	N/A
Software and Algorithms
Volocity	PerkinElmer	Version 6.0.1, RRID:SCR_002668
R	v.3.2.2	RRID: SCR_001905
TopHat2	v.2.0.12	RRID: SCR_013035
Cufflinks	v.2.2.1	RRID: SCR_014597
HISAT2	v.2.1.0	RRID: SCR_015530
HOMER	v.3.0	RRID: SCR_010881
Vienna RNA	v.2.4.3	RRID: SCR_008550
DAVID	v.6.8	RRID: SCR_001881
FIJI	Schindelin et al., 2012	Version 2.0.0-rc-65/1.51w, RRID:SCR_002285
Simple Neurite Tracer	Longair et al., 2011	https://imagej.net/Simple_Neurite_Tracer
MATLAB	Mathworks; v. R2016b	RRID:SCR_001622
GraphPad PRISM	Graphpad; v.5	RRID:SCR_002798
MaxQuant	v.1.4.1.2	RRID: SCR_014485
RapidSTORM	Wolter et al., 2012	N/A
PyMOL	https://www.pymol.org2/2	RRID:SCR_000305
Other
RNA-seq dataset	Shigeoka et al., 2016	GEO: GSE79352
RNA-seq dataset	Ding et al., 2017	GEO: GSM1948717
Proteome dataset	Cagnetta et al., 2018	PRIDE: PXD005469
POSTAR2 database	Zhu et al., 2019	N/A
Microfluidic chambers	Xona Microfluidics	Cat#SOC150
Falcon Cell Culture Inserts	Thermo Fisher Scientific	Cat#08-771-7/353102