Figure 4.

Separation performance of the device. Separation of target microbeads (10 µm) from a mixture of microbeads (1 and 3 µm) and bacteria (E. coli). Separation performance was measured based on the recovery and purity parameters defined in Figure 3b. The total infusion flow rate of the inlets (sample inlet and buffer inlet) was 500 µL/min (~0.4 cm/s flow speed), while the syringe pumps of the waste outlet had withdrawal rates of 400 µL/min (5× volumetric concentration), 450 µL/min (10× volumetric concentration), and 475 µL/min (20× volumetric concentration). The recovery rate and purity were determined by repeated experiments (n = 3).