Table 2.

Total phenolic yield (%), total phenol content (TPC) (mg gallic acid equivalents/100 g), oleuropein (Ole) content (mg/100 g), luteolin 7-O-glucoside (L7G) content (mg/100 g) and antioxidant activity (µmol trolox equivalents/100 g) of the ethanolic extracts as well as protein recovery (%) after alkaline extraction obtained by three different sequential extractions schemes.

Byproduct #	Scheme ‡	Phenolic Yield	TPC/Biomass	TPC/Extract	Ole/Biomass	Ole/Extract	L7G/Biomass	L7G/Extract	TEAC #/Biomass	TEAC #/Extract	Protein Recovery
OML	1	10.1 ± 0.2 b	454 ± 36 b	4476 ± 284 a	142 ± 3 ab	1395 ± 6 ab	31 ± 4 ab	309 ± 46 a	2024 ± 2 b	18,234 ± 139 b	11.2 ± 0.8 a
OML	2	11.7 ± 0.2 a	719 ± 89 a	6167 ± 847 a	79 ± 0.3 b	675 ± 6 b	28 ± 0.3 b	237 ± 6 b	3087 ± 143 a	25,459 ± 2809 a	13.7 ± 1.4 a
OML	3	11.7 ± 0.6 aA	585 ± 24 abB	4998 ± 65 aB	191 ± 45 aB	1790 ± 434 aB	38 ± 3 aB	338 ± 21 aB	2193 ± 69 bB	19,050 ± 101 bB	12.5 ± 0.8 a
OL	3	10.8 ± 0.8 A	1405 ± 99 A	13,108 ± 1877 A	1365 ± 6 A	12,694 ± 694 A	97 ± 1 A	903 ± 8 A	6364 ± 38 A	59,651 ± 6429 A	ND

^# OML, olive mill leaves; OL, olive leaves; TEAC, trolox equivalent antioxidant capacity. ^‡ Sequential extractions. Scheme 1: maceration with ethanol before protein extraction; Scheme 2: ethanolic extraction assisted by ultrasound after protein extraction; Scheme 3: ethanolic extraction assisted by ultrasound before protein extraction. In each column, data followed by the same minor letter are not statistically different from each other concerning the three schemes applied on OML, while data followed by the same capital letter are not statistically different from each other for the Scheme 3 applied in OML and OL (least significant difference test, p < 0.05).