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. 2019 Nov 18;55(11):747. doi: 10.3390/medicina55110747

Figure 3.

Figure 3

(a) Western blot analysis for TNF-alpha showed a downregulation of TNF-α expression in the pretreated groups ** p < 0.01, **** p  < 0.0001 vs. lipopolysaccharide (LPS); #### p < 0.0001 LPS + CBD 2.5 µM vs. LPS + CBD 5 µM. (b) Western blot analysis for IL-10 showed an increase in basal levels of the cytokine in the group pretreated with the CBG–CBD combination at 5 µM dose. **** p < 0.0001 vs. LPS. (c) Western blot analysis for IL-37 showed a statistically significant upregulation in groups pretreated with CBD alone and in association with CBG. **** p < 0.0001 vs. LPS. Blots are representative of three independent experiments. The densitometry analysis of bands representing means ± SEM of three independent experiments in which levels of each protein were normalized for glyceraldehyde 3-phosphate dehydrogenase (GAPDH).