Table 1.
Properties of skeletal myotubes expressing wild-type STIM1 or R429C.
| Control | Wild-type STIM1 | R429C | ||
|---|---|---|---|---|
| Width of myotubes | 1.00 ± 0.05 (40) | 1.02 ± 0.07 (40) | 0.95 ± 0.07 (40) | |
| SOCE | Peak area | 1.00 ± 0.15 (36) | 1.43 ± 0.18 * (36) | 1.04 ± 0.16 (39) |
| Slope (initial rate) | 1.00 ± 0.06 (30) | 1.02 ± 0.08 (30) | 0.81 ± 0.09 * (30) | |
| KCl response | 1.00 ± 0.07 (54) | 0.79 ± 0.08 * (54) | 1.20 ± 0.10 * (72) | |
| Caffeine response | 1.00 ± 0.07 (81) | 1.01 ± 0.11 (57) | 0.99 ± 0.05 (57) | |
| Cytosolic [Ca2+] (nM) | 84.37 ± 4.67 (69) | 85.84 ± 5.99 (70) | 101.19 ± 8.07 * (70) | |
| Ca2+ level in the SR | 1.00 ± 0.09 (50) | 1.00 ± 0.10 (50) | 0.80 ± 0.10 * (50) | |
| Total cellular Ca2+ level | 1.00 ± 0.09 (36) | 0.98 ± 0.09 (34) | 1.03 ± 0.11 (38) | |
The values, except for those of cytosolic [Ca2+], were normalized to the mean values of the controls. The values are presented as the mean ± s.e.m. for the number of myotubes shown in parentheses. *Significant difference compared with the control (p < 0.05).