Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 16;9:19215. doi: 10.1038/s41598-019-55635-4

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Altered channel properties of GluK2 and GluK4 mutants. (A) Top: Glutamate concentration-response curves for wild type GluK2 and GluK2/GluK4 receptors and the mutated GluK2/GluK2 K525E, GluK2/GluK4 Y555N and GluK2/GluK4 L825W subunit combinations. Points are mean % of maximum peak response ± SEM and curves are fits of the Hill equation. Bottom: Comparisons of pEC₅₀ for wild type and mutated GluK2 K525E, GluK4 Y555N, GluK4 L825W KARs. (B) Top: Two microelectrode voltage clamp (TEVC) traces for wild type and mutant GluK2 K525E, GluK4 Y555N, GluK4 L825W KARs in response to a 10-s application (bar) of 1 mM glutamate (for wild type and mutated GluK2 receptors) or 0.1 mM glutamate (for wild type and mutated GluK2/GluK4 receptors) at −80 mV. The responses have been scaled such that the peak responses are equal to aid comparison of the current decay kinetics, hence there are no vertical scale bars. Bottom: Comparisons of current decay τ1 values for wild type and mutated GluK2 K525E, GluK4 Y555N, GluK4 L825W KARs. Statistically significant differences are indicated by *(p < 0.05), **(p < 0.01) or ****(p < 0.0001). Abbreviations: Glu, glutamate; hGluK2, human GluK2; KA, kainic acid, pEC₅₀: negative logarithm of the EC₅₀ value.