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. 2019 Dec 16;9:19138. doi: 10.1038/s41598-019-55012-1

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Deconvolution of the amide I region of the Raman spectra of ex vivo porcine skin. (a) Unburnt skin, (b) Skin burnt at 200 °F for 10s, (c) Skin burnt at 200 °F for 30 s, (d) Skin burnt at 450 °F for 10 s, (e) Skin burnt at 450 °F for 30 s. The percentage of area under each curve is shown in bracket. Abbreviations: α – α-like helix, β – β sheets and β turns, dis – disordered structures, w – free and hydrogen bonded water, aa – amino acids. R²_adj = 0.99 for all the cases, indicating good fit, and the fitting curves are presented along with the data curve for visualization of the fitted results.