Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Dec 16;10:5719. doi: 10.1038/s41467-019-13671-8

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 3 — a H3K14ac levels were increased by hCG in a time-dependent manner. n = 3 biologically independent experiments. Data are presented as mean ± SEM, and different letters (a–d) indicate significant differences among groups (two-sided ANOVA and Holm–Šidák test), P (a, b) < 0.01, P (a, c) < 0.01, P (a, d) < 0.001, P (b, c) < 0.05, P (b, d) < 0.01, P (c, d) < 0.05. b Immunostaining revealed that LH increased H3K14ac (green) levels in GCs in vivo. Nuclei were dyed with Hoechst (blue). Scale bar = 100 µm. n = 3 biologically independent experiments. c ChIP-qPCR analysis of H3K14ac at the Areg promoter (−343 to −150) before and after the LH surge. P48: 48 h after PMSG treatment. H1–H4: 1–4 h after hCG treatment. Data are presented as mean ± SEM, and different letters (a–c) indicate significant differences among groups (two-sided ANOVA and Holm–Šidák test), P (a, b) < 0.01, P (a, c) < 0.001, P (b, c) < 0.001. d H3K14ac levels in Hdac3^flox/flox and Hdac3^cKO GCs were quantified by western blotting analysis. n = 3 biologically independent experiments. Data are presented as mean ± SEM. Asterisks (*) indicate significant differences at ***P < 0.001 determined by the two-sided t test. e ChIP-qPCR analysis of H3K14ac at the Areg promoter in Hdac3^flox/flox and Hdac3^cKO GCs. Data are presented as mean ± SEM, and different letters (a–c) indicate significant differences among groups (two-sided ANOVA and Holm–Šidák test), P (a, b) < 0.001, P (a, c) < 0.001, P (b, c) < 0.05. f Western blotting analysis showed that HDACi 4b (HDAC3 inhibitor) substantially increased H3K14ac levels. n = 3 biologically independent experiments. Data are presented as mean ± SEM. Asterisks (*) indicate significant differences at ***P < 0.001 determined by the two-sided t test. g ChIP-qPCR analysis of H3K14ac at the Areg promoter in control and HDACi 4b-treated GCs. Data are presented as mean ± SEM, and different letters (a–c) indicate significant differences among groups (two-sided ANOVA and Holm–Šidák test), P (a, b) < 0.001, P (a, c) < 0.001, P (b, c) < 0.001. Source data are provided as a Source Data file.