Skip to main content
. 2019 Dec 16;10(12):952. doi: 10.1038/s41419-019-2184-y

Fig. 2. Mitochondrial fission induces ciliogenesis.

Fig. 2

a Effects of mitochondrial fusion induced by Drp1 siRNA (siDrp1) treatment and fission by OPA1 siRNA (siOPA1) treatment. SH-SY5Y cells transfected with siDrp1 or siOPA1 were stained with a MitoTracker (white), ARL13B (green), and Hoechst 33342 dye (blue). b OPA1-knockout (KO) mouse embryonic fibroblasts (MEFs) and Drp1-KO MEFs were stained with a MitoTracker (white), ARL13B (green), and Hoechst 33342 dye (blue). c Effect of mitochondrial fusion on ciliogenesis in SH-SY5Y cells with siOPA1-enhanced ciliogenesis. Mitochondrial fusion was induced by siDrp1 transfection or Mdivi-1 (10 μM). Representative cilia images are presented. Cilia measurement data were obtained from about 200 cells per group and the experiments were repeated at least three times. Data are the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.005 vs. scrambled non-targeting siRNA (Sc) treatment or wild type (WT) MEF cells determined by ANOVA followed by a post hoc LSD test. Scale bar, 5 μm.