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. 2019 Dec 16;10(12):952. doi: 10.1038/s41419-019-2184-y

Fig. 5. Ciliogenesis mediates mitochondrial stress-induced autophagy.

Fig. 5

a, b SH-SY5Y cells transfected with siIFT88 were further treated with rotenone (200 nM) or MPP+ (5 mM) for 24 h. a Primary cilia were immunostained with ARL13B (green) and the nucleus was stained with Hoechst 33342 dye (blue). Cilia were measured in about 200 cells per group. b Mitochondria were stained with MitoTracker (white). Scale bar, 5 μm. c Inhibition of ciliogenesis with IFT88 siRNA (siIFT88) blocked rotenone- and MPP+-induced autophagy in SH-SY5Y cells. d SH-SY5Y/GFP-LC3 cells transfected with scrambled control siRNA (Sc) or siRNA against IFT88 (siIFT88) were further treated with rotenone (200 nM) and MPP+ (5 mM) for 24 h and then cells with autophagic punctate were counted under fluorescence microscopy. Scale bar, 10 μm. Data are the mean ± SEM. **p < 0.01, ***p < 0.005 between indicated groups determined by ANOVA followed by a post hoc LSD test.