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. 2019 Dec 16;2:470. doi: 10.1038/s42003-019-0662-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a–b Neutrophils were activated for 2 h by LPS (a) or ionomycin (b) with or without SP-D at two calcium concentrations and imaged after immunostaining for myeloperoxidase (MPO; green) and staining with DAPI for DNA (blue). MPO co-localized to NET-DNA upon stimulating neutrophils with LPS or ionomycin. a Pre-incubation of the indicated concentrations of SP-D with LPS showed the suppression of NETs release. b SP-D did not suppress NETosis induced by ionomycin (n = 2, with the whole experiment repeated twice, scale bar, 20 μm).