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. 2019 Dec 16;2:470. doi: 10.1038/s42003-019-0662-5

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Neutrophils were activated for 2 h with −ve control (media only), LPS or ionomycin with or without 20 and 40 μg/mL of SP-D. The cells were fixed and immunostained imaged for citH3 (red) and myeloperoxidase (MPO; green) and stained with DAPI for DNA-blue. LPS and ionomycin, but not buffer control, induced citH3 formation that co-localized with DNA and MPO. SP-D suppressed LPS-mediated, but not ionomycin-mediated citH3 formation (n = 2, scale bar, 40 μm).