Fig. 5. LPS-induced NETosis is high in the lungs of KO mice compared to WT mice.

Lung tissue sections were obtained from KO and WT mice that were instilled with either PBS or LPS. Tissue sections were processed, immunostained and imaged for citH3 (red) and myeloperoxidase (MPO; green) and stained with DAPI for DNA (blue). NETosis was absent in mice instilled with PBS. KO mice instilled with LPS exhibited more NETs in lung tissue, compared to the WT mice instilled also with LPS. (n = 2; scale bar, 23 μm).